Effects of infused glucose on glycogen metabolism in healthy humans

Détails

ID Serval
serval:BIB_83A3E6976345
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effects of infused glucose on glycogen metabolism in healthy humans
Périodique
Clinical Physiology
Auteur⸱e⸱s
Tounian  P., Schneiter  P., Henry  S., Tappy  L.
ISSN
0144-5979 (Print)
Statut éditorial
Publié
Date de publication
07/1996
Volume
16
Numéro
4
Pages
403-16
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
In order to determine whether or not hepatic glycogen breakdown contributes to systemic glucose flux during glucose infusion, net carbohydrate oxidation (indirect calorimetry) and the total rate of glucose appearance (6,6(-2)H-glucose) were measured in six healthy women during infusion of U-13 C labelled glucose (22 mumol/kg/min). Glucose infusion completely suppressed endogeneous glucose production and increased net carbohydrate oxidation from 10.9 +/- 1.6 to 18.9 +/- 1.0 mumol/kg/min. To differentiate between the oxidation of endogenous (i.e. glycogen) and of exogenous carbohydrates, the 13CO2 production was measured and the oxidation of exogenous 13C labelled carbohydrate was calculated. For this purpose, the specific recovery factor in breath of 13CO2 issued from oxidation of uniformly labelled glucose was determined during infusions of equimolar amounts of 13C bicarbonate, 1-13C acetate and 2-13C acetate. The average recovery was 53.9 +/- 1.5%. The oxidation of exogenous carbohydrate was 20.9 +/- 0.7 mumol/kg/min. This value was slightly higher than net carbohydrate oxidation, indicating that no oxidation of endogenous, unlabelled carbohydrate, and, hence, no utilization of hepatic glycogen took place. These results indicate that (i) estimation of glucose oxidation from indirect calorimetry and tracer technology give concordant results when an appropriate factor of 13CO2 recovery in breath is used, and (ii) utilization of previously formed glycogen is inhibited during hyperglycaemia and hyperinsulinaemia.
Mots-clé
Adult Blood Glucose/*metabolism Calorimetry, Indirect Carbohydrate Metabolism Carbon Radioisotopes/diagnostic use Fatty Acids/blood Female Glucagon/blood Gluconeogenesis/drug effects/*physiology Glucose/*administration & dosage Glycogen/*metabolism Humans Infusions, Intravenous Male Oxidation-Reduction/drug effects Reference Values
Pubmed
Web of science
Création de la notice
24/01/2008 14:36
Dernière modification de la notice
20/08/2019 15:43
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