Identification of swiprosin 1 in human lymphocytes

Détails

ID Serval
serval:BIB_8332A158BE62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of swiprosin 1 in human lymphocytes
Périodique
Proteomics
Auteur(s)
Vuadens  F., Rufer  N., Kress  A., Corthesy  P., Schneider  P., Tissot  J. D.
ISSN
1615-9853 (Print)
Statut éditorial
Publié
Date de publication
08/2004
Volume
4
Numéro
8
Pages
2216-20
Notes
Journal Article --- Old month value: Aug
Résumé
The aim of this work was to identify a new protein that discriminated CD8 from CD4 and CD19 lymphocytes. Proteins were separated by high-resolution two-dimensional electrophoresis. After silver staining, the gel images were captured with a laser densitometer, and studied with a dedicated software. This study confirmed the presence of two spots that appeared to be preferentially associated with CD8 lymphocytes, and mass spectrometry analyzes (liquid chromatography-tandem mass spectrometry, LC-MS/MS) identified six peptides for one spot and four for the other. The peptide sequences corresponded to an unknown protein that we named swiprosin 1 (Swiss-Prot Q96C19). Molecular analysis (reverse transcriptase-polymerase chain reaction, RT-PCR) and Northern blots confirmed that the gene expression was increased in purified populations of CD8 lymphocytes, when compared to CD19 and CD4 lymphocytes. Database mining revealed that swiprosin 1 contains two potential EF-hand domains, and therefore may have a role in calcium signaling. Its predominant presence in CD8 lymphocytes suggests that it may be involved in functions that are important for cytotoxic lymphocytes.
Mots-clé
Amino Acid Sequence Antigens, CD19/analysis Antigens, CD4/analysis CD8-Positive T-Lymphocytes/*chemistry/metabolism Calcium-Binding Proteins/*analysis/genetics Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Humans Mass Spectrometry Molecular Sequence Data Proteins/*analysis/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 16:34
Dernière modification de la notice
20/08/2019 15:43
Données d'usage