Ex Vivo Analysis of Kidney Graft Viability Using 31P Magnetic Resonance Imaging Spectroscopy.

Détails

ID Serval
serval:BIB_82D5D9F9154D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ex Vivo Analysis of Kidney Graft Viability Using 31P Magnetic Resonance Imaging Spectroscopy.
Périodique
Transplantation
Auteur(s)
Longchamp A., Klauser A., Songeon J., Agius T., Nastasi A., Ruttiman R., Moll S., Meier RPH, Buhler L., Corpataux J.M., Lazeyras F.
ISSN
1534-6080 (Electronic)
ISSN-L
0041-1337
Statut éditorial
Publié
Date de publication
09/2020
Peer-reviewed
Oui
Volume
104
Numéro
9
Pages
1825-1831
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The lack of organs for kidney transplantation is a growing concern. Expansion in organ supply has been proposed through the use of organs after circulatory death (donation after circulatory death [DCD]). However, many DCD grafts are discarded because of long warm ischemia times, and the absence of reliable measure of kidney viability. P magnetic resonance imaging (pMRI) spectroscopy is a noninvasive method to detect high-energy phosphate metabolites, such as ATP. Thus, pMRI could predict kidney energy state, and its viability before transplantation.
To mimic DCD, pig kidneys underwent 0, 30, or 60 min of warm ischemia, before hypothermic machine perfusion. During the ex vivo perfusion, we assessed energy metabolites using pMRI. In addition, we performed Gadolinium perfusion sequences. Each sample underwent histopathological analyzing and scoring. Energy status and kidney perfusion were correlated with kidney injury.
Using pMRI, we found that in pig kidney, ATP was rapidly generated in presence of oxygen (100 kPa), which remained stable up to 22 h. Warm ischemia (30 and 60 min) induced significant histological damages, delayed cortical and medullary Gadolinium elimination (perfusion), and reduced ATP levels, but not its precursors (AMP). Finally, ATP levels and kidney perfusion both inversely correlated with the severity of kidney histological injury.
ATP levels, and kidney perfusion measurements using pMRI, are biomarkers of kidney injury after warm ischemia. Future work will define the role of pMRI in predicting kidney graft and patient's survival.
Mots-clé
Adenosine Triphosphate/metabolism, Animals, Female, Kidney/metabolism, Kidney Transplantation, Magnetic Resonance Spectroscopy/methods, Perfusion, Swine
Pubmed
Web of science
Open Access
Oui
Financement(s)
Fonds national suisse / Carrières / PZ00P3-185927
Fonds national suisse / Projets / 320030_182658
Création de la notice
24/07/2020 14:24
Dernière modification de la notice
08/03/2021 7:26
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