Minigenes impart odorant receptor-specific axon guidance in the olfactory bulb.

Détails

ID Serval
serval:BIB_827808F693B3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Minigenes impart odorant receptor-specific axon guidance in the olfactory bulb.
Périodique
Neuron
Auteur⸱e⸱s
Vassalli A., Rothman A., Feinstein P., Zapotocky M., Mombaerts P.
ISSN
0896-6273 (Print)
ISSN-L
0896-6273
Statut éditorial
Publié
Date de publication
15/08/2002
Peer-reviewed
Oui
Volume
35
Numéro
4
Pages
681-696
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
An olfactory sensory neuron (OSN) expresses selectively one member from a repertoire of approximately 1000 odorant receptor (OR) genes and projects its axon to a specific glomerulus in the olfactory bulb. Both processes are here recapitulated by MOR23 and M71 OR minigenes, introduced into mice. Minigenes of 9 kb and as short as 2.2 kb are selectively expressed by neurons that do not coexpress the endogenous gene but coproject their axons to the same glomeruli. Deletion of a 395 bp upstream region in the MOR23 minigene abolishes expression. In this region we recognize sequence motifs conserved in many OR genes. Transgenic lines expressing the OR in ectopic epithelial zones form ectopic glomeruli, which also receive input from OSNs expressing the cognate endogenous receptor. This suggests a recruitment through homotypic interactions between OSNs expressing the same OR.
Mots-clé
Animals, Base Sequence/genetics, Binding Sites/genetics, Cell Differentiation/genetics, Gene Deletion, Gene Expression Regulation, Developmental/genetics, Genes, Reporter/genetics, Growth Cones/metabolism, Growth Cones/ultrastructure, Homeodomain Proteins/genetics, Introns/genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Olfactory Bulb/abnormalities, Olfactory Bulb/cytology, Olfactory Bulb/metabolism, Olfactory Pathways/abnormalities, Olfactory Pathways/cytology, Olfactory Pathways/metabolism, Olfactory Receptor Neurons/abnormalities, Olfactory Receptor Neurons/cytology, Olfactory Receptor Neurons/metabolism, Receptors, Odorant/genetics, Transgenes/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/10/2018 9:22
Dernière modification de la notice
25/05/2024 7:14
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