Breakdown of cytoskeletal proteins during meiosis of starfish oocytes and proteolysis induced by calpain.
Détails
ID Serval
serval:BIB_80BD2D7D0411
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Breakdown of cytoskeletal proteins during meiosis of starfish oocytes and proteolysis induced by calpain.
Périodique
Experimental Cell Research
ISSN
0014-4827[print], 0014-4827[linking]
Statut éditorial
Publié
Date de publication
2000
Volume
259
Numéro
1
Pages
117-126
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Meiosis reinitiation in starfish oocytes is characterized by Ca(2+) transients in the cytosol and in the nucleus and is accompanied by the disassembly of the nuclear envelope, a process which is likely to be mediated by the cleavage of selected proteins. We have used mass spectrometry analysis (mass profile fingerprinting) on 2D polyacrylamide gels of extracts of oocytes in which meiosis resumption was induced by 1-methyladenine and have identified five proteins that were specifically degraded: alpha-tubulin, lamin B, dynamin, and two kinds of actin. They are all components of the cytoskeleton or associated with it. We then investigated whether calpain, which is activated by the increase in cell Ca(2+), could cleave the same proteins that became degraded under the influence of 1-methyladenine and thus be involved in nuclear membrane breakdown. The investigation was prompted by the finding that microinjection of calpain into the nuclei of prophase arrested oocytes induced meiosis in the absence of 1-methyladenine. Incubation of prophase arrested (disrupted) oocytes with calpain produced a 2D gel protein pattern in which some of the degradation products coincided with those seen in oocytes challenged with 1-methyladenine.
Mots-clé
Actins/analysis, Actins/metabolism, Animals, Calcium/metabolism, Calpain/metabolism, Calpain/pharmacology, Cytoskeleton/metabolism, Dynamins, Electrophoresis, Gel, Two-Dimensional, GTP Phosphohydrolases/analysis, GTP Phosphohydrolases/metabolism, Lamin Type B, Lamins, Meiosis/drug effects, Meiosis/physiology, Microinjections, Nuclear Envelope/drug effects, Nuclear Envelope/metabolism, Nuclear Proteins/analysis, Nuclear Proteins/metabolism, Oocytes/cytology, Oocytes/enzymology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Starfish, Substrate Specificity/physiology, Tubulin/analysis, Tubulin/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 16:46
Dernière modification de la notice
20/08/2019 15:41