Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography.

Détails

ID Serval
serval:BIB_807895421137
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography.
Périodique
Scientific reports
Auteur⸱e⸱s
Shahmoradian S.H., Tsai EHR, Diaz A., Guizar-Sicairos M., Raabe J., Spycher L., Britschgi M., Ruf A., Stahlberg H., Holler M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
24/07/2017
Peer-reviewed
Oui
Volume
7
Numéro
1
Pages
6291
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
High-throughput three-dimensional cryogenic imaging of thick biological specimens is valuable for identifying biologically- or pathologically-relevant features of interest, especially for subsequent correlative studies. Unfortunately, high-resolution imaging techniques at cryogenic conditions often require sample reduction through sequential physical milling or sectioning for sufficient penetration to generate each image of the 3-D stack. This study represents the first demonstration of using ptychographic hard X-ray tomography at cryogenic temperatures for imaging thick biological tissue in a chemically-fixed, frozen-hydrated state without heavy metal staining and organic solvents. Applied to mammalian brain, this label-free cryogenic imaging method allows visualization of myelinated axons and sub-cellular features such as age-related pigmented cellular inclusions at a spatial resolution of ~100 nanometers and thicknesses approaching 100 microns. Because our approach does not require dehydration, staining or reduction of the sample, we introduce the possibility for subsequent analysis of the same tissue using orthogonal approaches that are expected to yield direct complementary insight to the biological features of interest.
Mots-clé
Animals, Brain/ultrastructure, Cryoelectron Microscopy/methods, Image Processing, Computer-Assisted/methods, Imaging, Three-Dimensional/methods, Mice, Mice, Inbred C57BL, Tomography, X-Ray/methods
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/06/2023 15:02
Dernière modification de la notice
08/07/2023 5:50
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