Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element.

Détails

ID Serval
serval:BIB_803A374889D3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Delavat F., Mitri S., Pelet S., van der Meer J.R.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
113
Numéro
24
Pages
E3375-E3383
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Horizontal gene transfer is an important evolutionary mechanism for bacterial adaptation. However, given the typical low transfer frequencies in a bacterial population, little is known about the fate and interplay of donor cells and the mobilized DNA during transfer. Here we study transfer of an integrative and conjugative element (ICE) among individual live bacterial cells. ICEs are widely distributed mobile DNA elements that are different than plasmids because they reside silent in the host chromosome and are maintained through vertical descent. Occasionally, ICEs become active, excise, and transmit their DNA to a new recipient, where it is reintegrated. We develop a fluorescent tool to differentiate excision, transfer, and reintegration of a model ICE named ICEclc (for carrying the clc genes for chlorocatechol metabolism) among single Pseudomonas cells by using time-lapse microscopy. We find that ICEclc activation is initiated in stationary phase cells, but excision and transfer predominantly occur only when such cells have been presented with new nutrients. Donors with activated ICE develop a number of different states, characterized by reduced cell division rates or growth arrest, persistence, or lysis, concomitant with ICE excision, and likely, ICE loss or replication. The donor cell state transitions can be described by using a stochastic model, which predicts that ICE fitness is optimal at low initiation rates in stationary phase. Despite highly variable donor cell fates, ICE transfer is remarkably robust overall, with 75% success after excision. Our results help to better understand ICE behavior and shed a new light on bacterial cellular differentiation during horizontal gene transfer.
Mots-clé
bacterial evolution, Pseudomonas, conjugation, modeling, time-lapse microscopy
Pubmed
Open Access
Oui
Création de la notice
08/07/2016 9:41
Dernière modification de la notice
20/08/2019 15:40
Données d'usage