Mouse tumor rejection antigens P815A and P815B: two epitopes carried by a single peptide

Détails

ID Serval
serval:BIB_7EED9A346ECD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mouse tumor rejection antigens P815A and P815B: two epitopes carried by a single peptide
Périodique
European Journal of Immunology
Auteur(s)
Lethe  B., van den Eynde  B., van Pel  A., Corradin  G., Boon  T.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
09/1992
Volume
22
Numéro
9
Pages
2283-8
Notes
Journal Article --- Old month value: Sep
Résumé
Mouse mastocytoma P815 expresses several distinct tumor rejection antigens recognized by syngeneic cytolytic T lymphocytes (CTL). Two of these tumor rejection antigens, P815A and P815B, are encoded by gene P1A, the sequence of which was reported previously. Tumor cell variants having lost one or both of these antigens were isolated by in vitro selection with CTL and also by collecting tumor cells that progressed in vivo after escaping a nearly complete immune rejection process. The structure of gene P1A in these antigen-loss variants was examined. Several A-B- variants presented a complete or partial deletion of the gene. One variant that had lost only antigen A displayed a point mutation in the first exon. Peptides were synthesized that corresponded to the normal sequence located in the region of this point mutation. They sensitized target cells to both anti-A and anti-B CTL. The homologous peptide encoded by the mutated gene of the P815 A-B+ variant sensitized cells only to anti-B CTL. We conclude that anti-A and anti-B CTL recognize on the same peptide two distinct epitopes that are affected differently by the mutation.
Mots-clé
Amino Acid Sequence Animals Antigens/*genetics/immunology Antigens, Neoplasm/*analysis Base Sequence *Epitopes Mast-Cell Sarcoma/*immunology Mice Molecular Sequence Data T-Lymphocytes, Cytotoxic/*immunology Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 14:39
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