Actions of aldosterone on polyadenylated ribonucleic acid and Na+ transport in the toad bladder
Détails
ID Serval
serval:BIB_7EBF66D8DE9C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Actions of aldosterone on polyadenylated ribonucleic acid and Na+ transport in the toad bladder
Périodique
Biochemistry
ISSN
0006-2960
Statut éditorial
Publié
Date de publication
09/1976
Peer-reviewed
Oui
Volume
15
Numéro
19
Pages
4279-85
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 21
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 21
Résumé
Polyadenylated ribonucleic acid [poly(A)(+)-RNA] has been isolated from the cytoplasm of the epithelium of the urinary bladder of the toad (Bufo marinus) by oligo-(deoxythymidylate)cellulose chromatography. Aldosterone increased the incorporation of [3H]uridine, and of [3H]uridine and [3H]adenosine (given in combination) into 7S, 12S, and 18S poly(A)(+)-RNA during the first 30 min of the action of the hormone, as defined by either a pulse or pulse-chase sequence. The quantity of cytoplasmic poly(A)(+)-RNA that hybridized to [3H]poly(uridine) was also increased by aldosterone. These results are consistent with the inference of induction of messenger RNA synthesis. This effect was most marked during the first 30 min of the action of the hormone.
Mots-clé
Aldosterone/*pharmacology
Animals
Biological Transport, Active
Bufo marinus
Cell Nucleus/drug effects/metabolism
Centrifugation, Density Gradient
Cytoplasm/drug effects/metabolism
Epithelium/drug effects/metabolism
Female
Poly A/*metabolism
RNA/*metabolism
Sodium/*metabolism
Urinary Bladder/drug effects/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 15:39