Direct visualization of supercoiled DNA molecules in solution.

Détails

ID Serval
serval:BIB_7DEBB007EB82
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Direct visualization of supercoiled DNA molecules in solution.
Périodique
EMBO Journal
Auteur⸱e⸱s
Adrian M., ten Heggeler-Bordier B., Wahli W., Stasiak A.Z., Stasiak A., Dubochet J.
ISSN
0261-4189[print], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
12/1990
Volume
9
Numéro
13
Pages
4551-4554
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The shape of supercoiled DNA molecules in solution is directly visualized by cryo-electron microscopy of vitrified samples. We observe that: (i) supercoiled DNA molecules in solution adopt an interwound rather than a toroidal form, (ii) the diameter of the interwound superhelix changes from about 12 nm to 4 nm upon addition of magnesium salt to the solution and (iii) the partition of the linking deficit between twist and writhe can be quantitatively determined for individual molecules.
Mots-clé
DNA, Superhelical/ultrastructure, Magnesium Chloride/pharmacology, Nucleic Acid Conformation, Solutions, Stereoisomerism
Pubmed
Web of science
Création de la notice
24/01/2008 10:25
Dernière modification de la notice
20/08/2019 14:39
Données d'usage