Topological locking restrains replication fork reversal.

Détails

ID Serval
serval:BIB_7D502D13B119
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Topological locking restrains replication fork reversal.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Fierro-Fernández M., Hernández P., Krimer D.B., Stasiak A., Schvartzman J.B.
ISSN
0027-8424[print], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
01/2007
Peer-reviewed
Oui
Volume
104
Numéro
5
Pages
1500-1505
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Two-dimensional agarose gel electrophoresis, psoralen cross-linking, and electron microscopy were used to study the effects of positive supercoiling on fork reversal in isolated replication intermediates of bacterial DNA plasmids. The results obtained demonstrate that the formation of Holliday-like junctions at both forks of a replication bubble creates a topological constraint that prevents further regression of the forks. We propose that this topological locking of replication intermediates provides a biological safety mechanism that protects DNA molecules against extensive fork reversals.
Mots-clé
Blotting, Southern, Cross-Linking Reagents/pharmacology, DNA/chemistry, DNA Repair, DNA, Bacterial/chemistry, DNA, Superhelical/chemistry, Electrophoresis, Gel, Two-Dimensional/methods, Escherichia coli/metabolism, Ficusin/chemistry, Intercalating Agents/pharmacology, Microscopy, Electron/methods, Nucleic Acid Conformation, Nucleic Acid Hybridization, Plasmids/metabolism, Temperature
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:36
Dernière modification de la notice
20/08/2019 14:38
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