Erythropoietin receptor expression is concordant with erythropoietin but not with common beta chain expression in the rat brain throughout the life span

Détails

ID Serval
serval:BIB_7D4E9F80E4C7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Erythropoietin receptor expression is concordant with erythropoietin but not with common beta chain expression in the rat brain throughout the life span
Périodique
J Comp Neurol
Auteur(s)
Sanchez P. E., Navarro F. P., Fares R. P., Nadam J., Georges B., Moulin C., Le Cavorsin M., Bonnet C., Ryvlin P., Belmeguenai A., Bodennec J., Morales A., Bezin L.
ISSN
1096-9861 (Electronic)
ISSN-L
0021-9967
Statut éditorial
Publié
Date de publication
2009
Volume
514
Numéro
4
Pages
403-14
Langue
anglais
Notes
Sanchez, Pascal E
Navarro, Fabrice P
Fares, Raafat P
Nadam, Jeremie
Georges, Beatrice
Moulin, Colette
Le Cavorsin, Marion
Bonnet, Chantal
Ryvlin, Philippe
Belmeguenai, Amor
Bodennec, Jacques
Morales, Anne
Bezin, Laurent
eng
Research Support, Non-U.S. Gov't
J Comp Neurol. 2009 Jun 1;514(4):403-14. doi: 10.1002/cne.22020.
Résumé
Brain effects of erythropoietin (Epo) are proposed to involve a heteromeric receptor comprising the classical Epo receptor (Epo-R) and the common beta chain (betac). However, data documenting the pattern of betac gene expression in the healthy brain, in comparison with that of the Epo-R gene, are still lacking. The present study is the first to investigate at the same time betac, Epo-R, and Epo gene expression within different rat brain areas throughout the life span, from neonatal to elderly stages, using quantitative RT-PCR for transcripts. Corresponding proteins were localized by using immunohistochemistry. We demonstrate that the betac transcript level does not correlate with that of Epo-R or Epo, whereas the Epo-R transcript level strongly correlates with that of Epo throughout the life span in all brain structures analyzed. Both Epo and Epo-R were detected primarily in neurons. In the hippocampus, the greatest Epo-R mRNA levels were measured during the early postnatal period and in middle-aged rats, associated with an intense neuronal immunolabeling. Conversely, betac protein was barely detectable in the brain at all ages, even in neurons expressing high levels of Epo-R. Finally, betac transcript could not be detected in PC12 cells, even after nerve growth factor-induced neuritogenesis, which is a condition that dramatically enhances Epo-R transcript level. Altogether, our data suggest that most neurons are likely to express high levels of Epo-R but low, if not null, levels of betac. Given that Epo protects extended populations of neurons after injury, a yet-to-be-identified receptor heterocomplex including Epo-R may exist in the large population of brain neurons that does not express betac.
Mots-clé
Aging/metabolism, Analysis of Variance, Animals, Astrocytes/metabolism, Brain/*growth & development/*metabolism, Erythropoietin/*metabolism, Gene Expression, Immunohistochemistry, Male, Microglia/metabolism, Neurites/metabolism, Neurons/metabolism, PC12 Cells, RNA, Messenger/metabolism, Rats, Rats, Sprague-Dawley, Receptors, Erythropoietin/*metabolism, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Création de la notice
29/11/2018 12:37
Dernière modification de la notice
20/08/2019 14:38
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