Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster.

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Ressource 1Télécharger: BIB_7CEF4F60D81A.P001.pdf (1543.37 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_7CEF4F60D81A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster.
Périodique
European Journal of Neuroscience
Auteur(s)
Ignatious Raja J.S., Katanayeva N., Katanaev V.L., Galizia C.G.
ISSN
1460-9568 (Electronic)
ISSN-L
0953-816X
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
39
Numéro
8
Pages
1245-1255
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we show that inhibiting Go in sensory neurons by pertussis toxin leads to behavioral deficits. We heterologously expressed the olfactory receptor dOr22a in human embryonic kidney cells (HEK293T). Stimulation with an odor led to calcium influx, which was amplified via calcium release from intracellular stores. Subsequent experiments indicated that the signaling was mediated by the Gβγ subunits of the heterotrimeric Go/i proteins. Finally, using in vivo calcium imaging, we show that Go and Gi contribute to odor responses both for the fast (phasic) as for the slow (tonic) response component. We propose a transduction cascade model involving several parallel processes, in which the metabotropic component is activated by Go and Gi , and uses Gβγ.
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/05/2014 16:54
Dernière modification de la notice
20/10/2020 8:19
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