Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line

Détails

ID Serval
serval:BIB_7BF8D6D63212
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Shaw  P., Bovey  R., Tardy  S., Sahli  R., Sordat  B., Costa  J.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
05/1992
Volume
89
Numéro
10
Pages
4495-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 15
Résumé
A wild-type p53 gene under control of the metallothionein MT-1 promoter was stably transfected into human colon tumor-derived cell line EB. Repeated inductions of the metallothionein wild-type p53 gene with zinc chloride results in progressive detachment of wild-type p53 cells grown on culture dishes. Examination at both the light and electron microscopic level revealed that cells expressing wild-type p53 developed morphological features of apoptosis. DNA from both attached and detached cells was degraded into a ladder of nucleosomal-sized fragments. Expression of wild-type p53 inhibited colony formation in soft agar and tumor formation in nude mice. Furthermore, established tumors in nude mice underwent regression if wild-type p53 expression was subsequently induced. Regressing tumors showed histological features of apoptosis. Thus, regression of these tumors was the result of apoptosis occurring in vivo. Apoptosis may be a normal part of the terminal differentiation program of colonic epithelial cells. Our results suggest that wild-type p53 could play a critical role in this process.
Mots-clé
Base Sequence Cell Death/*genetics Cell Line Colonic Neoplasms/*genetics/pathology/ultrastructure DNA, Neoplasm/genetics/metabolism Gene Expression *Genes, p53 Humans Kinetics Metallothionein/genetics Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction/methods Promoter Regions (Genetics) RNA, Messenger/genetics/metabolism RNA, Neoplasm/genetics/metabolism Transcription, Genetic *Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:51
Dernière modification de la notice
20/08/2019 14:37
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