The MHC reactivity of the T cell repertoire prior to positive and negative selection.

Détails

ID Serval
serval:BIB_7BE62904395A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The MHC reactivity of the T cell repertoire prior to positive and negative selection.
Périodique
Cell
Auteur⸱e⸱s
Zerrahn J., Held W., Raulet D.H.
ISSN
0092-8674
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
88
Numéro
5
Pages
627-636
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
T cell antigen receptors (TCRs) on mature T cells react with peptide antigens presented by self-MHC proteins and also frequently cross-react with foreign MHC proteins. The fundamental question whether MHC reactivity is inherent in the germline TCR sequences or is imposed by thymic selection was addressed here by inducing nonselective maturation of immature thymocytes in the absence of MHC molecules. MHC reactivity in the preselection repertoire is very high, but no higher than in the normal repertoire. Cross-reactivity of clones with multiple MHC molecules occurred to a similar extent in the preselection and MHC-selected repertoires. The results establish the MHC reactivity of the germline TCR repertoire, indicate the minimum fraction of immature thymocytes that must undergo negative selection, and suggest that some TCR-MHC contacts may be conserved.
Mots-clé
Animals, Antibodies, Monoclonal/pharmacology, CD4-Positive T-Lymphocytes/chemistry, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/chemistry, CD8-Positive T-Lymphocytes/immunology, Female, Flow Cytometry, Gene Expression/physiology, Genes, MHC Class I/immunology, Genes, MHC Class II/immunology, Hybridomas, Immunophenotyping, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Organ Culture Techniques, Receptors, Antigen, T-Cell/immunology, Receptors, Antigen, T-Cell/metabolism, Thymus Gland/cytology, Thymus Gland/embryology
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 16:24
Dernière modification de la notice
20/08/2019 15:37
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