Neuropeptide Y: a novel angiogenic factor from the sympathetic nerves and endothelium
Détails
ID Serval
serval:BIB_7AEEBE594B3A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Neuropeptide Y: a novel angiogenic factor from the sympathetic nerves and endothelium
Périodique
Circulation Research
ISSN
0009-7330 (Print)
Statut éditorial
Publié
Date de publication
07/1998
Volume
83
Numéro
2
Pages
187-95
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 27
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 27
Résumé
Sympathetic nerves have long been suspected of trophic activity, but the nature of their angiogenic factor has not been determined. Neuropeptide Y (NPY), a sympathetic cotransmitter, is the most abundant peptide in the heart and the brain. It is released during nerve activation and ischemia and causes vasoconstriction and smooth muscle cell proliferation. Here we report the first evidence that NPY is angiogenic. At low physiological concentrations, in vitro, it promotes vessel sprouting and adhesion, migration, proliferation, and capillary tube formation by human endothelial cells. In vivo, in a murine angiogenic assay, NPY is angiogenic and is as potent as a basic fibroblast growth factor. The NPY action is specific and is mediated by Y1 and Y2 receptors. The expression of both receptors is upregulated during cell growth; however, Y2 appears to be the main NPY angiogenic receptor. Its upregulation parallels the NPY-induced capillary tube formation on reconstituted basement membrane (Matrigel); the Y2 agonist mimics the tube-forming activity of NPY, whereas the Y2 antagonist blocks it. Endothelium contains not only NPY receptors but also peptide itself, its mRNA, and the "NPY-converting enzyme" dipeptidyl peptidase IV (both protein and mRNA), which terminates the Y1 activity of NPY and cleaves the Tyr1-Pro2 from NPY to form an angiogenic Y2 agonist, NPY3-36. Endothelium is thus not only the site of action of NPY but also the origin of the autocrine NPY system, which, together with the sympathetic nerves, may be important in angiogenesis during tissue development and repair.
Mots-clé
Animals
Antigens, CD26/biosynthesis/genetics/physiology
Aorta/drug effects
Capillaries
Collagen/pharmacology
Drug Combinations
Endothelial Growth Factors/pharmacology
Endothelium, Vascular/*chemistry
Female
Fibroblast Growth Factor 2/pharmacology
Gene Expression
Humans
Laminin/pharmacology
Lymphokines/pharmacology
Male
Mice
Mice, Inbred C57BL
Neovascularization, Physiologic/*drug effects
Neuropeptide Y/biosynthesis/genetics/isolation &
purification/metabolism/pharmacology/*physiology
Peptide Fragments/metabolism/pharmacology
Polymerase Chain Reaction
Proteoglycans/pharmacology
RNA, Messenger/biosynthesis
Rats
Rats, Sprague-Dawley
Receptors, Neuropeptide Y/biosynthesis/drug effects/genetics/physiology
Sympathetic Nervous System/*chemistry
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Pubmed
Web of science
Création de la notice
25/01/2008 11:55
Dernière modification de la notice
20/08/2019 15:36