Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor
Détails
ID Serval
serval:BIB_7AC909A1305B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor
Périodique
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
06/1986
Volume
83
Numéro
12
Pages
4223-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Complement component C9 contains two distinct cysteine-rich domains exhibiting high sequence resemblance to a domain present in the low density lipoprotein (LDL) receptor and epidermal growth factor precursor, respectively. Antibodies were raised against a peptide corresponding to the most conserved region of the LDL receptor/C9-homology segment. The antibodies were shown by immunoblotting to bind specifically to C9 but also to crossreact with C8 alpha, the alpha subunit of complement component C8. Moreover, a monoclonal antibody to a neoantigen present in polymerized C9 bound to an epitope exposed on C8 within the C5b-8 complex but buried in monomeric C8, suggesting that C8 and C9 undergo similar conformational changes during membrane-attack-complex assembly. Isolated C8 alpha-gamma exhibited the propensity to polymerize in the presence of Zn2+ and urea, as already demonstrated for C9. These data indicate that C8 alpha is closely related, both structurally and functionally, to C9.
Mots-clé
Complement C8/*immunology
Complement C9/*immunology
Complement Membrane Attack Complex
Complement System Proteins/immunology
Cross Reactions
Cysteine
Epitopes
Humans
Macromolecular Substances
Oligopeptides/chemical synthesis/immunology
Receptors, LDL/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:18
Dernière modification de la notice
20/08/2019 15:36