Low-temperature pausing of cultivated mammalian cells.

Détails

ID Serval
serval:BIB_7AA9F3187CB8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Low-temperature pausing of cultivated mammalian cells.
Périodique
Biotechnology and Bioengineering
Auteur(s)
Hunt L., Hacker D.L., Grosjean F., De Jesus M., Uebersax L., Jordan M., Wurm F.M.
ISSN
0006-3592 (Print)
ISSN-L
0006-3592
Statut éditorial
Publié
Date de publication
2005
Volume
89
Numéro
2
Pages
157-163
Langue
anglais
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a variety of cultivation vessels. High cell viability and exponential growth were observed after pausing a recombinant Chinese hamster ovary cell line (CHO-Clone 161) in a temperature range of 6-24 degrees C in microcentrifuge tubes for up to 3 weeks. After pausing in T-flasks at 4 degrees C for 9 days, adherent cultures of CHO-DG44 and human embryonic kidney (HEK293 EBNA) cells resumed exponential growth when incubated at 37 degrees C. Adherent cultures of CHO-DG44 cells paused for 2 days at 4 degrees C in T-flasks and suspension cultures of HEK293 EBNA cells paused for 3 days at either 4 degrees C or 24 degrees C in spinner flasks were efficiently transfected by the calcium phosphate-DNA coprecipitation method, yielding reporter protein levels comparable to those from nonpaused cultures. Finally, cultures of a recombinant CHO cell line (CHO-YIgG3) paused for 3 days at 4 degrees C, 12 degrees C, or 24 degrees C in bioreactors achieved the same cell mass and recombinant protein productivity levels as nonpaused cultures. The success of this approach to cell storage with rodent and human cell lines points to a general biological phenomenon which may have a wide range of applications for cultivated mammalian cells.
Mots-clé
Animals, Bioreactors, CHO Cells, Cell Culture Techniques/methods, Cell Line, Cell Proliferation, Cell Survival/physiology, Cricetinae, Cricetulus, Cryopreservation/methods, Humans, Kidney/physiology, Temperature, Time Factors
Pubmed
Web of science
Création de la notice
06/03/2015 12:48
Dernière modification de la notice
20/08/2019 15:36
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