Use of a combined ex vivo/in vivo population approach for screening of human genes involved in the human immunodeficiency virus type 1 life cycle for variants influencing disease progression.

Détails

ID Serval
serval:BIB_79F3606839DF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of a combined ex vivo/in vivo population approach for screening of human genes involved in the human immunodeficiency virus type 1 life cycle for variants influencing disease progression.
Périodique
Journal of virology
Auteur⸱e⸱s
Bleiber G., May M., Martinez R., Meylan P., Ott J., Beckmann J.S., Telenti A.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
79
Numéro
20
Pages
12674-80
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. - Publication Status: ppublish
Résumé
Humans differ substantially with respect to susceptibility to human immunodeficiency virus type 1 (HIV-1). We evaluated variants of nine host genes participating in the viral life cycle for their role in modulating HIV-1 infection. Alleles were assessed ex vivo for their impact on viral replication in purified CD4 T cells from healthy blood donors (n = 128). Thereafter, candidate alleles were assessed in vivo in a cohort of HIV-1-infected individuals (n = 851) not receiving potent antiretroviral therapy. As a benchmark test, we tested 12 previously reported host genetic variants influencing HIV-1 infection as well as single nucleotide polymorphisms in the nine candidate genes. This led to the proposition of three alleles of PML, TSG101, and PPIA as potentially associated with differences in progression of HIV-1 disease. In a model considering the combined effects of new and previously reported gene variants, we estimated that their effect might be responsible for lengthening or shortening by up to 2.8 years the period from 500 CD4 T cells/mul to <200 CD4 T cells/mul.
Mots-clé
Alleles, CD4-Positive T-Lymphocytes, Cells, Cultured, Genetic Predisposition to Disease, HIV Infections, HIV-1, Humans, Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:17
Dernière modification de la notice
20/08/2019 14:36
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