Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis.

Détails

ID Serval
serval:BIB_79D422FC5178
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis.
Périodique
Journal of Bacteriology
Auteur⸱e⸱s
Veening J.W., Kuipers O.P., Brul S., Hellingwerf K.J., Kort R.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
2006
Volume
188
Numéro
8
Pages
3099-3109
Langue
anglais
Résumé
The spore-forming bacterium Bacillus subtilis is able to form highly organized multicellular communities called biofilms. This coordinated bacterial behavior is often lost in domesticated or laboratory strains as a result of planktonic growth in rich media for many generations. However, we show here that the laboratory strain B. subtilis 168 is still capable of forming spatially organized multicellular communities on minimal medium agar plates, exemplified by colonies with vein-like structures formed by elevated bundles of cells. In line with the current model for biofilm formation, we demonstrate that overproduction of the phosphorelay components KinA and Spo0A stimulates bundle formation, while overproduction of the transition state regulators AbrB and SinR leads to repression of formation of elevated bundles. Time-lapse fluorescence microscopy studies of B. subtilis green fluorescent protein reporter strains show that bundles are preferential sites for spore formation and that flat structures surrounding the bundles contain vegetative cells. The elevated bundle structures are formed prior to sporulation, in agreement with a genetic developmental program in which these processes are sequentially activated. Perturbations of the phosphorelay by disruption and overexpression of genes that lead to an increased tendency to sporulate result in the segregation of sporulation mutations and decreased heat resistance of spores in biofilms. These results stress the importance of a balanced control of the phosphorelay for biofilm and spore development.
Mots-clé
Adaptation, Physiological, Bacillus subtilis/genetics, Bacillus subtilis/physiology, Bacterial Proteins/genetics, Bacterial Proteins/physiology, Biofilms/growth & development, DNA-Binding Proteins/genetics, DNA-Binding Proteins/physiology, Gene Deletion, Genes, Reporter, Green Fluorescent Proteins/analysis, Green Fluorescent Proteins/genetics, Microscopy, Fluorescence, Morphogenesis, Photomicrography, Protein Kinases/genetics, Protein Kinases/physiology, Signal Transduction, Spores, Bacterial/physiology, Staining and Labeling, Transcription Factors/genetics, Transcription Factors/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 15:25
Dernière modification de la notice
20/08/2019 14:36
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