Antagonism of catecholamine receptor signaling by expression of cytoplasmic domains of the receptors.
Détails
ID Serval
serval:BIB_79099B3E78DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Antagonism of catecholamine receptor signaling by expression of cytoplasmic domains of the receptors.
Périodique
Science
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
1993
Volume
259
Numéro
5100
Pages
1453-1457
Langue
anglais
Résumé
The actions of many hormones and neurotransmitters are mediated by the members of a superfamily of receptors coupled to heterotrimeric guanine nucleotide-binding proteins (G proteins). These receptors are characterized by a highly conserved topographical arrangement in which seven transmembrane domains are connected by intracellular and extracellular loops. The interaction between these receptors and G proteins is mediated in large part by the third intracellular loop of the receptor. Coexpression of the third intracellular loop of the alpha 1B-adrenergic receptor with its parent receptor inhibited receptor-mediated activation of phospholipase C. The inhibition extended to the closely related alpha 1C-adrenergic receptor subtype, but not the phospholipase C-coupled M1 muscarinic acetylcholine receptor nor the adenylate cyclase-coupled D1A dopamine receptor. These results suggest that the receptor-G protein interface may represent a target for receptor antagonist drugs.
Mots-clé
Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, Cyclic AMP/metabolism, Cytoplasm/metabolism, GTP-Binding Proteins/metabolism, Globins/genetics, Glutathione Transferase/genetics, Glutathione Transferase/metabolism, Humans, Inositol Phosphates/metabolism, Kinetics, Molecular Sequence Data, Muscarinic Antagonists, Oligodeoxyribonucleotides, Plasmids, Protein Structure, Secondary, Receptors, Adrenergic, alpha/genetics, Receptors, Adrenergic, alpha/metabolism, Receptors, Dopamine D1/antagonists & inhibitors, Receptors, Dopamine D1/genetics, Receptors, Muscarinic/genetics, Receptors, Muscarinic/metabolism, Recombinant Fusion Proteins/metabolism, Signal Transduction, Transfection, Type C Phospholipases/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 12:05
Dernière modification de la notice
20/08/2019 15:35