Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets
Détails
ID Serval
serval:BIB_77711925A8EA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets
Périodique
European Journal of Immunology
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
02/1982
Peer-reviewed
Oui
Volume
12
Numéro
2
Pages
166-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
The expression at the cell surface level of two previously defined human Ia antigen subsets, NG1 and NG2, was studied. Two monoclonal hybridoma antibodies, D1-12 (anti-NG1) and D4-22 (anti-NG2), labeled with distinct fluorochromes, were used as probes. The results indicate that NG1 and NG2 molecules are simultaneously expressed on the same cell, and moreover, that they are identifiable as spatially separate structures. In addition, when a series of 6 other anti-Ia monoclonal antibodies previously described were analyzed in a checkerboard test of binding inhibition at the cell surface level, they were found to closely mimic the reactivity pattern of either D1-12 or D4-22 antibody, suggesting that NG1 and NG2 Ia subsets express independent clusters of highly immunogenic epitopes.
Mots-clé
Antibodies, Monoclonal/immunology
Antigen-Antibody Reactions
B-Lymphocytes/immunology
Cell Line
*Epitopes
Histocompatibility Antigens Class II/*immunology
Humans
Pubmed
Web of science
Création de la notice
20/01/2008 16:55
Dernière modification de la notice
20/08/2019 15:34