A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

Détails

ID Serval
serval:BIB_77507EE2FBC3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae
Périodique
International Journal of Leprosy and Other Mycobacterial Diseases
Auteur⸱e⸱s
Honore  N., Perrani  E., Telenti  A., Grosset  J., Cole  S. T.
ISSN
0148-916X (Print)
Statut éditorial
Publié
Date de publication
12/1993
Volume
61
Numéro
4
Pages
600-4
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
Mots-clé
Alleles Amino Acid Sequence Base Sequence DNA Primers/chemistry DNA, Bacterial/chemistry DNA, Single-Stranded/chemistry DNA-Directed RNA Polymerases/chemistry/*genetics Dapsone/therapeutic use Drug Resistance, Microbial/genetics Drug Therapy, Combination Genes, Bacterial Humans Leprosy, Lepromatous/drug therapy/*microbiology Male Molecular Sequence Data Mutation Mycobacterium leprae/*drug effects/genetics Polymerase Chain Reaction Polymorphism, Genetic Prothionamide/therapeutic use Recurrence Rifampin/*pharmacology/therapeutic use
Pubmed
Web of science
Création de la notice
25/01/2008 14:46
Dernière modification de la notice
20/08/2019 14:34
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