A novel and simple procedure for determining T cell epitopes in protein antigens

Détails

ID Serval
serval:BIB_76A0AD75131B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A novel and simple procedure for determining T cell epitopes in protein antigens
Périodique
Journal of Immunological Methods
Auteur⸱e⸱s
Demotz  S., Matricardi  P., Lanzavecchia  A., Corradin  G.
ISSN
0022-1759 (Print)
Statut éditorial
Publié
Date de publication
08/1989
Volume
122
Numéro
1
Pages
67-72
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 15
Résumé
A human T cell epitope was mapped on tetanus toxin by the use of a simple method. Tetanus toxin peptide preparations were produced by various specific chemical and proteolytic cleavages. T cell proliferative response to these peptide preparations was assayed to determine which amino acid residues were present or absent in the T cell epitope. Cyanogen bromide-derived tetanus toxin peptides were separated by electrophoresis and transferred onto nitrocellulose. T cell proliferative response to nitrocellulose fragments was assayed to determine the molecular weight of the antigenic tetanus toxin peptide. The combination of the two procedures allowed us to define a 21-mer tetanus toxin peptide recognized by the T cell clone. This method represents an easily performed alternative for the mapping of T cell epitopes on protein antigen.
Mots-clé
CD4-Positive T-Lymphocytes/*immunology Cell Line Electrophoresis, Polyacrylamide Gel *Epitopes Humans Lymphocyte Activation Peptide Fragments/*immunology T-Lymphocytes, Helper-Inducer/*immunology Tetanus Toxin/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 14:33
Données d'usage