Identification of a new cancer/testis gene family, CT47, among expressed multicopy genes on the human X chromosome.

Détails

ID Serval
serval:BIB_738898667BD1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of a new cancer/testis gene family, CT47, among expressed multicopy genes on the human X chromosome.
Périodique
Genes, Chromosomes and Cancer
Auteur⸱e⸱s
Chen Y.T., Iseli C., Venditti C.A., Old L.J., Simpson A.J., Jongeneel C.V.
ISSN
1045-2257[print], 1045-2257[linking]
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
45
Numéro
4
Pages
392-400
Langue
anglais
Résumé
Cancer/testis (CT) genes are normally expressed in germ cells only, yet are reactivated and expressed in some tumors. Of the approximately 40 CT genes or gene families identified to date, 20 are on the X chromosome and are present as multigene families, many with highly conserved members. This indicates that novel CT gene families may be identified by detecting duplicated expressed genes on chromosome X. By searching for transcript clusters that map to multiple locations on the chromosome, followed by in silico analysis of their gene expression profiles, we identified five novel gene families with testis-specific expression and >98% sequence identity among family members. The expression of these genes in normal tissues and various tumor cell lines and specimens was evaluated by qualitative and quantitative RT-PCR, and a novel CT gene family with at least 13 copies was identified on Xq24, designated as CT47. mRNA expression of CT47 was found mainly in the testes, with weak expression in the placenta. Brain tissue was the only positive somatic tissue tested, with an estimated CT47 transcript level 0.09% of that found in testis. Among the tumor specimens tested, CT47 expression was found in approximately 15% of lung cancer and esophageal cancer specimens, but not in colorectal cancer or breast cancer. The putative CT47 protein consists of 288 amino acid residues, with a C-terminus rich in alanine and glutamic acid. The only species other than human in which a gene homologous to CT47 has been detected is the chimpanzee, with the predicted protein showing approximately 80% identity in its carboxy terminal region.
Mots-clé
Cell Line, Tumor, Chromosomes, Human, X/genetics, Expressed Sequence Tags, Female, Gene Dosage, Gene Expression Profiling, Genes, Neoplasm, Humans, Male, Multigene Family, Neoplasms/genetics, Proteins/genetics, Reverse Transcriptase Polymerase Chain Reaction, Testis/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:39
Dernière modification de la notice
20/08/2019 14:31
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