Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives

Détails

ID Serval
serval:BIB_731E034526DE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives
Périodique
Pharmaceutical Research
Auteur⸱e⸱s
Johansen  P., Men  Y., Audran  R., Corradin  G., Merkle  H. P., Gander  B.
ISSN
0724-8741 (Print)
Statut éditorial
Publié
Date de publication
07/1998
Volume
15
Numéro
7
Pages
1103-10
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
PURPOSE: Tetanus toxoid (Ttxd) encapsulated in polyester microspheres (MS) for single injection immunization have so far given pulsatile in vitro release and strong immune response in animals, but no boosting effect. This has been ascribed to insufficient toxoid stability within the MS exposed to in vivo conditions over a prolonged time period. This study examined the effect of co-encapsulated putative stabilizing additives. METHODS: Two different Ttxd were encapsulated in poly(D,L-lactic-co-glycolic acid) (PLGA 50:50) and poly(D,L-lactic acid) (PLA) MS by spray-drying. The influence of co-encapsulated additives on toxoid stability, loading in and release from the MS, was studied by fluorimetry and ELISA. RESULTS: Co-encapsulated albumin, trehalose and gamma-hydroxypropyl cyclodextrin all improved the toxoid encapsulation efficiency in PLGA 50:50 MS. Albumin increased the encapsulation efficiency of antigenic Ttxd by one to two orders of magnitude. Further, with albumin or a mixture of albumin and trehalose ELISA responsive Ttxd was released over 1-2 months following a pulsatile pattern. CONCLUSIONS: Optimized Ttxd containing MS may be valuable for a single-dose vaccine delivery system.
Mots-clé
Capsules Chemistry, Pharmaceutical Drug Stability Kinetics Lactic Acid/administration & dosage/chemistry Microspheres Pharmaceutic Aids/*administration & dosage/*chemistry Polyglycolic Acid/administration & dosage/chemistry Polymers/administration & dosage/chemistry Tetanus Toxoid/*administration & dosage/*chemistry/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 14:31
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