Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton
Détails
ID Serval
serval:BIB_722E71E40A49
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton
Périodique
Neuron
ISSN
0896-6273 (Print)
Statut éditorial
Publié
Date de publication
10/2004
Volume
44
Numéro
2
Pages
321-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 14
Research Support, Non-U.S. Gov't --- Old month value: Oct 14
Résumé
We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding to the actin cytoskeleton. Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses. This hyperspinogenesis was accompanied by enhanced actin polymerization and spine motility. Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility.
Mots-clé
Actins/*physiology
Amino Acid Sequence
Animals
Cytoskeleton/*physiology
Dendrites/*physiology/ultrastructure
Microfilament Proteins/*genetics/metabolism
Microscopy, Electron
Models, Neurological
Molecular Sequence Data
Mutation
Nerve Tissue Proteins/*genetics/metabolism
Neuronal Plasticity
Patch-Clamp Techniques
Pyramidal Cells/*cytology
Rats
Sequence Homology
Synapses/physiology/ultrastructure
Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:26
Dernière modification de la notice
20/08/2019 14:30