High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays.

Détails

ID Serval
serval:BIB_71E34713FAA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays.
Périodique
Nature biomedical engineering
Auteur⸱e⸱s
Brandenberg N., Hoehnel S., Kuttler F., Homicsko K., Ceroni C., Ringel T., Gjorevski N., Schwank G., Coukos G., Turcatti G., Lutolf M.P.
ISSN
2157-846X (Electronic)
ISSN-L
2157-846X
Statut éditorial
Publié
Date de publication
09/2020
Peer-reviewed
Oui
Volume
4
Numéro
9
Pages
863-874
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell culture in solid extracellular matrices hampers the routine use of the organoids at the industrial scale. Here, we report the fabrication of microengineered cell culture devices and scalable and automated methods for suspension culture and real-time analysis of thousands of individual gastrointestinal organoids trapped in microcavity arrays within a polymer-hydrogel substrate. The absence of a solid matrix substantially reduces organoid heterogeneity, which we show for mouse and human gastrointestinal organoids. We use the devices to screen for anticancer drug candidates with patient-derived colorectal cancer organoids, and apply high-content image-based phenotypic analyses to reveal insights into mechanisms of drug action. The scalable organoid-culture technology should facilitate the use of organoids in drug development and diagnostics.
Mots-clé
Animals, Cell Aggregation, Cell Culture Techniques/methods, Cells, Cultured, Dimethylpolysiloxanes/chemistry, Drug Evaluation, Preclinical, High-Throughput Screening Assays, Humans, Hydrogels/chemistry, Intestines/cytology, Mice, Organogenesis, Organoids/cytology, Organoids/drug effects, Organoids/growth & development, Stem Cells/cytology
Pubmed
Web of science
Création de la notice
10/06/2020 15:49
Dernière modification de la notice
23/11/2020 7:24
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