Generation of cytolytic T lymphocytes in vitro. X. Induction of primary and secondary CTL responses by the mitogen sodium periodate

Détails

ID Serval
serval:BIB_7171F06AC6C9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Generation of cytolytic T lymphocytes in vitro. X. Induction of primary and secondary CTL responses by the mitogen sodium periodate
Périodique
Journal of Immunology
Auteur⸱e⸱s
Maryanski  J. L., Cerottini  J. C., Brunner  K. T.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
01/1979
Volume
122
Numéro
1
Pages
342-9
Notes
Journal Article --- Old month value: Jan
Résumé
Short-term (15 min) sodium periodate (NaIO4) treatment of mouse spleen cells previously primed in vivo or in vitro against alloantigens induced the formation of secondary (2 degree) cytolytic T lymphocytes (CTL) specific for the priming antigens. CTL formation was readily demonstrable within 24 hr after treatment.This early CTL response occurred equally well in the presence or absence of cytosine arabinoside (Ara C), indicating that NaIO4 could induce CTL independently of DNA synthesis. Forty-eight hours after periodate treatment, the lytic activity was similar to that observed in parallel cultures stimulated with irradiated allogeneic spleen cells, although the peak activity was reached earlier (day 4) and was somewhat lower than that induced by alloantigen. The addition of irradiated NaIO4-treated unprimed syngeneic spleen cells to cultures of untreated alloimmune spleen cells also led to CTL formation, which suggests an indirect mechanism of activation. In contrast to alloimmune spleen cells, normal spleen cells treated with NaIO4 developed only very low levels of cytotoxicity after 4 days of incubation. However, in the presence of PHA, such cells were capable of lysing syngeneic and allogeneic target cells.
Mots-clé
Animals Cells, Cultured *Cytotoxicity, Immunologic DNA/biosynthesis Epitopes Female Immunologic Memory Isoantigens Kinetics Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred DBA Mitogens/*pharmacology Periodic Acid/*pharmacology Spleen/immunology/radiation effects T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 11:14
Dernière modification de la notice
20/08/2019 14:29
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