Lentiviral vectors: a powerful tool to target astrocytes in vivo.

Détails

Ressource 1Télécharger: 5_24020977_Postprint.pdf (1580.15 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_7104A620BE7E
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Lentiviral vectors: a powerful tool to target astrocytes in vivo.
Périodique
Current Drug Targets
Auteur(s)
Delzor A., Escartin C., Déglon N.
ISSN
1873-5592 (Electronic)
ISSN-L
1389-4501
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
14
Numéro
11
Pages
1336-1346
Langue
anglais
Notes
Publication types: Journal Article ; Review Publication Status: ppublish
Résumé
The morphological and functional diversity of astrocytes, and their essential contribution in physiological and pathological conditions, are starting to emerge. However, experimental systems to investigate neuron-glia interactions and develop innovative approaches for the treatment of central nervous system (CNS) disorders are still very limited. Fluorescent reporter genes have been used to visualize populations of astrocytes and produce an atlas of gene expression in the brain. Knock-down or knock-out of astrocytic proteins using transgenesis have also been developed, but these techniques remain complex and time-consuming. Viral vectors have been developed to overexpress or silence genes of interest as they can be used for both in vitro and in vivo studies in adult mammalian species. In most cases, high transduction efficiency and long-term transgene expression are observed in neurons but there is limited expression in astrocytes. Several strategies have been developed to shift the tropism of lentiviral vectors (LV) and allow local and controlled gene expression in glial cells. In this review, we describe how modifications of the interaction between the LV envelope glycoprotein and the surface receptor molecules on target cells, or the integration of cell-specific promoters and miRNA post-transcriptional regulatory elements have been used to selectively express transgenes in astrocytes.
Mots-clé
Animals, Astrocytes/physiology, Central Nervous System Diseases/physiopathology, Central Nervous System Diseases/therapy, Gene Expression Regulation, Gene Targeting/methods, Genetic Vectors, Humans, Lentivirus/genetics, Lentivirus/physiology, MicroRNAs/genetics, MicroRNAs/metabolism, Neurons/physiology, Transduction, Genetic, Transgenes, Viral Tropism, Virus Internalization
Pubmed
Web of science
Création de la notice
24/10/2013 16:53
Dernière modification de la notice
20/08/2019 14:29
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