Staphylococcal biofilm formation on the surface of three different calcium phosphate bone grafts: a qualitative and quantitative in vivo analysis.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_700F280BF335
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Staphylococcal biofilm formation on the surface of three different calcium phosphate bone grafts: a qualitative and quantitative in vivo analysis.
Périodique
Journal of Materials Science. Materials in Medicine
Auteur(s)
Furustrand Tafin U., Betrisey B., Bohner M., Ilchmann T., Trampuz A., Clauss M.
ISSN
1573-4838 (Electronic)
ISSN-L
0957-4530
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
26
Numéro
3
Pages
130
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Differences in physico-chemical characteristics of bone grafts to fill bone defects have been demonstrated to influence in vitro bacterial biofilm formation. Aim of the study was to investigate in vivo staphylococcal biofilm formation on different calcium phosphate bone substitutes. A foreign-body guinea-pig infection model was used. Teflon cages prefilled with β-tricalcium phosphate, calcium-deficient hydroxyapatite, or dicalcium phosphate (DCP) scaffold were implanted subcutaneously. Scaffolds were infected with 2 × 10(3) colony-forming unit of Staphylococcus aureus (two strains) or S. epidermidis and explanted after 3, 24 or 72 h of biofilm formation. Quantitative and qualitative biofilm analysis was performed by sonication followed by viable counts, and microcalorimetry, respectively. Independently of the material, S. aureus formed increasing amounts of biofilm on the surface of all scaffolds over time as determined by both methods. For S. epidermidis, the biofilm amount decreased over time, and no biofilm was detected by microcalorimetry on the DCP scaffolds after 72 h of infection. However, when using a higher S. epidermidis inoculum, increasing amounts of biofilm were formed on all scaffolds as determined by microcalorimetry. No significant variation in staphylococcal in vivo biofilm formation was observed between the different materials tested. This study highlights the importance of in vivo studies, in addition to in vitro studies, when investigating biofilm formation of bone grafts.
Mots-clé
Animals, Biofilms, Bone Transplantation, Calcium Phosphates/administration & dosage, Guinea Pigs, Staphylococcus/metabolism, Surface Properties, Tissue Scaffolds
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/04/2015 18:22
Dernière modification de la notice
20/08/2019 14:28
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