Secretion of ACTH, LPH and beta-endophin from human pituitary tumours in vitro

Détails

ID Serval
serval:BIB_6F93DAE2C431
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Secretion of ACTH, LPH and beta-endophin from human pituitary tumours in vitro
Périodique
Clinical Endocrinology
Auteur⸱e⸱s
Gillies  G., Ratter  S., Grossman  A., Gaillard  R., Lowry  P. J., Besser  G. M., Rees  L. H.
ISSN
0300-0664 (Print)
Statut éditorial
Publié
Date de publication
08/1980
Volume
13
Numéro
2
Pages
197-205
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
Basal and stimulated secretion of immunoreactive ACTH, LPH and beta-endorphin from four human pituitary tumours has been studied in vitro using a superfused, isolated cell system. Chromatography of cell secretions under acid-dissociating conditions demonstrated that the human tumor cells released immunoreactive peptides with the elution profiles of alpha h (1-39) ACTH, beta h-LPH, gamma h-LPH and beta h-endorphin confirming that beta h-endorphin is secreted by human pituitary tumour cells and is not formed by enzymic cleavage from beta h-LPH in blood. No alpha- or beta h-MSH, nor any higher molecular weight forms of ACTH or LPH were detected. The cells from all four tumours responded to stimulation with rat stalk-median eminence extract (SME) and synthetic AVP with a concomitant release of ACTH, beta-LPH, gamma-LPH and beta-endorphin. In contrast to the isolated rat anterior pituitary cells, the pattern of responses to SME and AVP were indistinguishable and the release provoked by rat SME could be accounted for virtually entirely by its vasopressin content. No stimulation of release was observed when the cells were exposed to a variety of biogenic amines. Addition of hydrocortisone to the perfusion buffer of two tumours resulted in a slow inhibition of both basal and stimulated ACTH and LPH release. These data demonstrate that human pituitary tumour tissue from patients with Cushing's disease and Nelson's syndrome can be studied in vitro and that such studies may contribute to a greater understanding of the aetiology of these diseases.
Mots-clé
Adrenocorticotropic Hormone/*secretion Arginine Vasopressin/pharmacology Cell Separation Chromatography, Gel Endorphins/*secretion Humans Median Eminence/physiology Pituitary Gland, Anterior/secretion Pituitary Neoplasms/*secretion Secretory Rate/drug effects beta-Lipotropin/*secretion
Pubmed
Web of science
Création de la notice
15/02/2008 16:57
Dernière modification de la notice
20/08/2019 14:28
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