Entamoeba histolytica zymodemes: exhibition of gamma and delta bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium

Détails

ID Serval
serval:BIB_6F5921672DD9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Entamoeba histolytica zymodemes: exhibition of gamma and delta bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium
Périodique
Experimental Parasitology
Auteur(s)
Blanc  D., Sargeaunt  P. G.
ISSN
0014-4894
Statut éditorial
Publié
Date de publication
01/1991
Peer-reviewed
Oui
Volume
72
Numéro
1
Pages
87-90
Notes
Journal Article --- Old month value: Jan
Résumé
Entamoeba histolytica isolated from human can be associated with either symptomatic disease or with asymptomatic carriers. Pathogenic and nonpathogenic strains can be distinguished on the basis of differences in the electrophoretic patterns of four isoenzymes (zymodeme). With glucose phosphate isomerase and phosphoglucomutase, we observed variation of the expression of their gamma bands as a function of starch volume in culture. We cultured E. histolytica strains from different zymodemes in Robinson medium using both a low (2-4 mg/bottle) and a high (12-15 mg/bottle) content of rice starch as supplement. These cultures were monitored by electrophoresis of glucose phosphate isomerase and phosphoglucomutase. Strains having gamma or delta bands exhibited those bands when a high content of starch was used in culture, but did not do so with a low content. Contrarily, strains that never exhibited those bands did not express them when the amount of starch in the culture was increased. However, alpha and beta bands of the same isoenzymes were always present and never showed any variation. The results suggest that expression of gamma and delta bands of glucose phosphate isomerase and phosphoglucomutase are subject to culture conditions and that genes coding for those isoenzymes may be different from those coding for the alpha and beta bands.
Mots-clé
Animals Culture Media Entamoeba histolytica/classification/*enzymology Glucose-6-Phosphate Isomerase/*analysis Isoenzymes/*analysis Phosphoglucomutase/*analysis Starch/*metabolism
Pubmed
Web of science
Création de la notice
29/01/2008 15:20
Dernière modification de la notice
20/08/2019 14:28
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