Monoclonal antibodies against carcinoembryonic antigen (CEA) used in a solid-phase enzyme immunoassay. First clinical results.

Détails

ID Serval
serval:BIB_6F177F3439FB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Monoclonal antibodies against carcinoembryonic antigen (CEA) used in a solid-phase enzyme immunoassay. First clinical results.
Périodique
Journal of Immunological Methods
Auteur(s)
Buchegger F., Phan M., Rivier D., Carrel S., Accolla R.S., Mach J.P.
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Statut éditorial
Publié
Date de publication
1982
Volume
49
Numéro
2
Pages
129-139
Langue
anglais
Résumé
A solid-phase enzyme immunoassay using both mouse monoclonal and goat polyclonal antibodies against carcinoembryonic antigen (CEA) was developed. The assay detects 0.6 to 1.2 ng of CEA per ml of serum and has 3 incubation steps which can be performed in 1 day. Polystyrene balls coated with polyclonal goat anti-CEA antibodies are first incubated with heat-extracted serum samples. Bound CEA is then detected by addition of mouse monoclonal antibodies, followed by goat IgG anti-mouse IgG1 coupled to alkaline phosphatase. Results with this enzyme immunoassay using monoclonal antibodies (M-EIA) have been compared with those obtained by the conventional inhibition radioimmunoassay (RIA) using goat antiserum. Three hundred and eighty serum samples from 167 patients with malignant or non-malignant diseases and from 134 normal individuals with or without heavy smoking habits were analyzed by the 2 assays. Excellent correlation between the results of the 2 assays was obtained, but the M-EIA, using monoclonal antibodies from a single hybridoma, did not discriminate better than the conventional RIA between CEA produced by different types of carcinoma and between CEA associated with malignant or non-malignant diseases. Follow-up studies of several patients by sequential CEA determinations with the 2 assays showed that the M-EIA was as accurate as the RIA for the detection of tumor recurrences.
Mots-clé
Animals, Antibodies, Monoclonal/immunology, Binding Sites, Antibody, Breast Neoplasms/analysis, Breast Neoplasms/immunology, Carcinoembryonic Antigen/analysis, Carcinoembryonic Antigen/immunology, Colonic Neoplasms/analysis, Colonic Neoplasms/immunology, Female, Goats, Humans, Immunoenzyme Techniques, Kinetics, Liver Cirrhosis/immunology, Liver Neoplasms/analysis, Liver Neoplasms/immunology, Male, Mice, Mice, Inbred Strains, Polystyrenes, Radioimmunoassay, Rectal Neoplasms/analysis, Rectal Neoplasms/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 11:27
Dernière modification de la notice
20/08/2019 14:28
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