Allelotype analysis of mouse lung carcinomas reveals frequent allelic losses on chromosome 4 and an association between allelic imbalances on chromosome 6 and K-ras activation

Détails

ID Serval
serval:BIB_6EDCE9B8ADA6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Allelotype analysis of mouse lung carcinomas reveals frequent allelic losses on chromosome 4 and an association between allelic imbalances on chromosome 6 and K-ras activation
Périodique
Cancer Research
Auteur⸱e⸱s
Hegi  M. E., Devereux  T. R., Dietrich  W. F., Cochran  C. J., Lander  E. S., Foley  J. F., Maronpot  R. R., Anderson  M. W., Wiseman  R. W.
ISSN
0008-5472 (Print)
Statut éditorial
Publié
Date de publication
12/1994
Volume
54
Numéro
23
Pages
6257-64
Notes
Journal Article --- Old month value: Dec 1
Résumé
We generated allelotypes of 38 methylene chloride-induced lung carcinomas from female C57BL/6J x C3H/6J F1 (hereafter called B6C3F1) mice. Two or more polymorphic markers per autosome, most of them microsatellites, were examined for loss of heterozygosity. Allelic losses throughout the genome were generally infrequent except for markers on chromosome 4, which were lost in approximately one-half of the carcinomas. Analysis of lung adenomas indicated that chromosome 4 loss was associated with malignant conversion. In addition, chromosome 4 loss were specific for lung carcinomas based on comparison to methylene chloride-induced liver tumors and additional studies of lung tumors from a variety of treatment protocols and different mouse strains. Preferential loss of the maternal chromosome 4 was observed in B6C3F1 carcinomas. Analyses of additional tumors induced in mice from two reciprocal crosses, A/J x C3H/HeJ F1 (hereafter called AC3F1) and C3H/HeJ x A/J F1 (hereafter called C3AF1), provided evidence for the inactivation of one allele of the putative chromosome 4 tumor suppressor gene by parental imprinting. Most B6C3F1 tumors lost all chromosome 4 markers examined, suggesting nondisjunction events. In contrast, several C3AF1 and AC3F1 tumors appeared to have interstitial deletions that defined the smallest region of overlap as a 9-cM interval between Ifa-2 and D4Nds2. The homologous region on human chromosome 9p21-22 is frequently lost in a variety of tumors including lung cancers. A candidate tumor suppressor gene, MTS1, is located in this region, which is homozygously deleted or mutated in cell lines derived from a variety of human tumors. Finally, an association between K-ras gene activation and allelic imbalances on chromosome 6 was observed for B6C3F1 lung tumors.
Mots-clé
*Alleles Animals *Chromosome Deletion Female *Gene Expression Regulation, Neoplastic *Genes, ras Lung Neoplasms/chemically induced/*genetics Male Methylene Chloride Mice Mutation
Pubmed
Web of science
Création de la notice
25/01/2008 13:06
Dernière modification de la notice
20/08/2019 14:28
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