Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_6EC8C68163D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis
Périodique
Chemical Science
Auteur(s)
Zhu Y.D., Qiao L., Prudent M., Bondarenko A., Gasilova N., Moller S.B., Lion N., Pick H., Gong T.Q., Chen Z.X., Yang P.Y., Loveyf L.T., Girault H.H
ISSN
2041-6539
ISSN-L
2041-6520
Statut éditorial
Publié
Date de publication
2016
Volume
7
Numéro
5
Pages
2897-2995
Langue
anglais
Résumé
Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and accurately identify bacteria in human blood samples by combining optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and efficient immunoaffinity enrichment/separation. A library of bacteria reference mass spectra at different cell numbers was firstly built. Due to a reduced sample spot size, the reference spectra could be obtained from as few as 10 to 10(2) intact bacterial cells. Bacteria in human blood samples were then extracted using antibodies-modified magnetic beads for MS fingerprinting. By comparing the sample spectra with the reference spectra based on a cosine correlation, bacteria with concentrations as low as 500 cells per mL in blood serum and 8000 cells per mL in whole blood were identified. The proposed method was further applied to positive clinical blood cultures (BCs) provided by a local hospital, where Escherichia coli and Staphylococcus aureus were identified. Because of the method's high sensitivity, the BC time required for diagnosis can be greatly reduced. As a proof of concept, whole blood spiked with a low initial concentration (10(2) or 10(3) cells per mL) of bacteria was cultured in commercial BC bottles and analysed by the developed method after different BC times. Bacteria were successfully identified after 4 hours of BC. Therefore, an entire diagnostic process could be accurately accomplished within half a day using the newly developed method, which could facilitate the timely determination of appropriate anti-bacterial therapy and decrease the risk of mortality from bloodstream infections.
Web of science
Open Access
Oui
Création de la notice
27/05/2016 9:43
Dernière modification de la notice
30/04/2021 6:11
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