Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.

Détails

ID Serval
serval:BIB_6E1DFE2C7433
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.
Périodique
Vox Sanguinis
Auteur(s)
Rubin O., Crettaz D., Canellini G., Tissot J.D., Lion N.
ISSN
1423-0410[electronic], 0042-9007[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
95
Numéro
4
Pages
288-297
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
BACKGROUND AND OBJECTIVES: Microparticles (MPs) are small phospholipid vesicles of less than 1 microm, shed in blood flow by various cell types. These MPs are involved in several biological processes and diseases. MPs have also been detected in blood products; however, their role in transfused patients is unknown. The purpose of this study was to characterize those MPs in blood bank conditions. MATERIALS AND METHODS: Qualitative and quantitative experiments using flow cytometry or proteomic techniques were performed on MPs derived from erythrocytes concentrates. In order to count MPs, they were either isolated by various centrifugation procedures or counted directly in erythrocyte concentrates. RESULTS: A 20-fold increase after 50 days of storage at 4 degrees C was observed (from 3370 +/- 1180 MPs/microl at day 5 to 64 850 +/- 37 800 MPs/microl at day 50). Proteomic analysis revealed changes of protein expression comparing MPs to erythrocyte membranes. Finally, the expression of Rh blood group antigens was shown on MPs generated during erythrocyte storage. CONCLUSIONS: Our work provides evidence that storage of red blood cell is associated with the generation of MPs characterized by particular proteomic profiles. These results contribute to fundamental knowledge of transfused blood products.
Mots-clé
Blood Banks/methods, Blood Preservation/adverse effects, Cell-Derived Microparticles, Erythrocyte Transfusion/adverse effects, Erythrocytes/cytology, Flow Cytometry, Humans, Proteomics
Pubmed
Web of science
Création de la notice
25/02/2009 15:09
Dernière modification de la notice
20/08/2019 14:27
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