In vivo imaging of colitis and colon cancer development in mice using high resolution chromoendoscopy

Détails

ID Serval
serval:BIB_6D996BDCC3F6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo imaging of colitis and colon cancer development in mice using high resolution chromoendoscopy
Périodique
Gut
Auteur⸱e⸱s
Becker  C., Fantini  M. C., Wirtz  S., Nikolaev  A., Kiesslich  R., Lehr  H. A., Galle  P. R., Neurath  M. F.
ISSN
0017-5749 (Print)
Statut éditorial
Publié
Date de publication
2005
Volume
54
Numéro
7
Pages
950-954
Notes
PT - Journal Article
Résumé
BACKGROUND: Mouse models of colitis and cancer are indispensable for our understanding of the pathogenesis of these diseases. In the past, mice had to be sacrificed in order to analyse colitis activity and tumour development. We have developed a safe method for high resolution endoscopic monitoring of living mice. METHODS: Mice developing colitis or colonic tumours were anaesthetised using avertine and repeatedly examined by endoscopy. A novel miniendoscope (1.9 mm outer diameter), denoted Coloview, was introduced via the anus and the colon was carefully insufflated with an air pump before analysis of the colonic mucosa. An extra working channel allowed the introduction of biopsy forceps or injection needles as well as surface staining with methylene blue in order to visualise the surface of the crypts and the pit pattern architecture. RESULTS: Endoscopic pictures obtained were of high quality and allowed monitoring and grading of disease. Scoring of colitis activity as well as tumour size and growth was possible. In addition, pit pattern analysis using chromoendoscopy permitted discrimination between inflammatory and neoplastic changes. Biopsies yielded enough tissue for molecular and histopathological analyses. CONCLUSIONS: In summary, chromoendoscopy in mice allows monitoring of the development of colitis and colon cancer with high resolution. Manipulations such as local injection of reagents or taking biopsies can be performed easily
Mots-clé
Animals/Azoxymethane/Cell Transformation,Neoplastic/Pathology/Colitis/chemically induced/complications/Colonic Neoplasms/etiology/Colonoscopes/Colonoscopy/methods/Dextran Sulfate/Disease Models,Animal/Disease Progression/Intestinal Mucosa/Mice/Mice,Inbred Strains/Severity of Illness Index
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 19:35
Dernière modification de la notice
20/08/2019 15:27
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