Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.

Détails

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Etat: Public
Version: Final published version
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ID Serval
serval:BIB_6D940CF0A59D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Périodique
The Journal of cell biology
Auteur(s)
Marek M., Vincenzetti V., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
01/06/2020
Peer-reviewed
Oui
Volume
219
Numéro
6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Sterols are crucial components of biological membranes, which are synthetized in the ER and accumulate in the plasma membrane (PM). Here, by applying a genetically encoded sterol biosensor (D4H), we visualize a sterol flow between PM and endosomes in the fission yeast Schizosaccharomyces pombe. Using time-lapse and correlative light-electron microscopy, we found that inhibition of Arp2/3-dependent F-actin assembly promotes the reversible relocalization of D4H from the PM to internal sterol-rich compartments (STRIC) labeled by synaptobrevin Syb1. Retrograde sterol internalization to STRIC is independent of endocytosis or an intact Golgi, but depends on Ltc1, a LAM/StARkin-family protein localized to ER-PM contact sites. The PM in ltc1Δ cells over-accumulates sterols and upon Arp2/3 inhibition forms extended ER-interacting invaginations, indicating that sterol transfer contributes to PM size homeostasis. Anterograde sterol movement from STRIC is independent of canonical vesicular trafficking but requires Arp2/3, suggesting a novel role for this complex. Thus, transfer routes orthogonal to vesicular trafficking govern the flow of sterols in the cell.
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/04/2020 17:15
Dernière modification de la notice
11/11/2020 7:09
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