Laser-capture microdissection.

Détails

ID Serval
serval:BIB_6CA8735D22A2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Laser-capture microdissection.
Périodique
Nature Protocols
Auteur⸱e⸱s
Espina V., Wulfkuhle J.D., Calvert V.S., VanMeter A., Zhou W., Coukos G., Geho D.H., Petricoin E.F., Liotta L.A.
ISSN
1750-2799 (Electronic)
ISSN-L
1750-2799
Statut éditorial
Publié
Date de publication
2006
Volume
1
Numéro
2
Pages
586-603
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, RNA transcript profiling, cDNA library generation, proteomics discovery and signal-pathway profiling. Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1-1.5 h.
Mots-clé
Cell Separation/instrumentation, Cell Separation/methods, DNA, Infrared Rays, Lasers, Microdissection/instrumentation, Microdissection/methods, Proteins, RNA, Staining and Labeling, Ultraviolet Rays
Pubmed
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
20/08/2019 15:26
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