Effect of N-nitroso-N-methylurethane on gas exchange, lung compliance, and surfactant function of rabbits

Détails

ID Serval
serval:BIB_6C6A65853C7E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effect of N-nitroso-N-methylurethane on gas exchange, lung compliance, and surfactant function of rabbits
Périodique
Intensive Care Medicine
Auteur⸱e⸱s
Pappert  D., Gilliard  N., Heldt  G., Merritt  T. A., Wagner  P. D., Spragg  R. G.
ISSN
0342-4642 (Print)
Statut éditorial
Publié
Date de publication
04/1996
Volume
22
Numéro
4
Pages
345-52
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
OBJECTIVES: To define the effect of N-nitroso-N-methyl-urethane (NNNMU) on pulmonary gas exchange, compliance and the biochemical and functional properties of the lung surfactant system. DESIGN: Four days after inducing lung injury, gas exchange and pulmonary compliance were studied and a bronchoalveolar lavage was taken. SETTING: Experimental laboratory of a university department of medicine, division of pulmonary and critical care medicine. ANIMALS: Ten rabbits after they had received an injection of NNNMU and five control animals. INTERVENTIONS: Controlled mechanical ventilation and bronchoalveolar lavage. MEASUREMENTS AND RESULTS: Measurements of gas exchange (using the multiple inert gas elimination technique), hemodynamics and pulmonary compliance were performed during ventilatory and hemodynamic steady state. A bronchoalveolar lavage (BAL) was taken after sacrificing the animal. BAL samples were processed for cell count and biochemical and functional surfactant analysis. Animals injected with NNNMU developed mild, but significant reduction in PaO2, while maintaining eucapnia during spontaneous air breathing. V/Q distributions and arterial blood gases were similar in all animals when ventilated mechanically with a fixed tidal volume. Compliance of the lung and phospholipid levels in lavage of NNNMU animals was significantly lower than in control animals (CON). Function of surfactant recovered from animals receiving NNNMU was decreased significantly where compared to CON. Thus, NNNMU resulted in a lowered lavage surfactant phospholipid content, impaired surfactant function, decreased compliance and hypoxemia during spontaneous ventilation. However, gas exchange was similar to that of control animals during mechanical ventilation. CONCLUSION: We conclude that NNNMU-induced gas exchange abnormalities present after 4 days are mild and are reversed by fixed volume mechanical ventilation despite marked alteration in surfactant function and lung compliance. These observations further define properties of a lung injury model that is of value in the study of surfactant replacement.
Mots-clé
Animals Blood Gas Analysis Bronchoalveolar Lavage Hemodynamic Processes/drug effects Lung Compliance/*drug effects Nitrosomethylurethane/pharmacology Pulmonary Gas Exchange/*drug effects Pulmonary Surfactants/*drug effects/physiology Rabbits Respiratory Distress Syndrome, Adult/*physiopathology
Pubmed
Web of science
Création de la notice
28/01/2008 11:50
Dernière modification de la notice
20/08/2019 15:26
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