Computerized microassay of keratinocyte cell-plastic attachment and proliferation for assessing net stimulatory, inhibitory and toxic effects of compounds on nonimmortalized cell lines
Détails
ID Serval
serval:BIB_6BF4948AEB89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Computerized microassay of keratinocyte cell-plastic attachment and proliferation for assessing net stimulatory, inhibitory and toxic effects of compounds on nonimmortalized cell lines
Périodique
Skin Pharmacology
ISSN
1011-0283 (Print)
Statut éditorial
Publié
Date de publication
1993
Volume
6
Numéro
2
Pages
135-47
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Résumé
Testing of pharmacological agents that affect growth of epidermal keratinocytes (EK) requires a standardized assay. We have developed an assay measuring net effects of stimulatory (e.g. growth factors), inhibitory (e.g. methotrexate) or toxic (e.g. Triton X-100) compounds. The amount of crystal violet staining viable EK attached to the wells of standard 96-well microplates is measured in situ using an ELISA plate reader. Optical density readings are directly converted into cell counts by computer software. Counts obtained by this method strongly correlate with the results obtained using the [3H]thymidine uptake assay and direct cell counts. The assay standardizes measurements of nonimmortalized EK lines with different innate proliferative properties and allows accurate quantitation of EK numbers in the range of 2,500-500,000 EK/well.
Mots-clé
Cell Adhesion/*drug effects
Cell Count/*methods
Cell Division/*drug effects
Cell Line
Epidermal Growth Factor/pharmacology
Gentian Violet/diagnostic use
Humans
Keratinocytes/*drug effects
Methotrexate/pharmacology
Microcomputers
Octoxynol
Polyethylene Glycols/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 17:30
Dernière modification de la notice
20/08/2019 15:26