Brain spectrin(240/235) and brain spectrin(240/235E): conservation of structure and location within mammalian neural tissue.

Détails

ID Serval
serval:BIB_6BD17AB64889
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Brain spectrin(240/235) and brain spectrin(240/235E): conservation of structure and location within mammalian neural tissue.
Périodique
Brain Research Bulletin
Auteur⸱e⸱s
Riederer B.M., Lopresti L.L., Krebs K.E., Zagon I.S., Goodman S.R.
ISSN
0361-9230 (Print)
ISSN-L
0361-9230
Statut éditorial
Publié
Date de publication
1988
Volume
21
Numéro
4
Pages
607-616
Langue
anglais
Résumé
We demonstrate that the brain spectrin isoforms (240/235) and (240/235E) are present in all mammalian species studied (human, bovine, mouse, and rat). Immunohistochemistry with a panel of eleven polyclonal antibodies have indicated an identical localization of the brain spectrin isoforms in all mammalian species. Brain spectrin(240/235) is found primarily in axons, and brain spectrin(240/235E) primarily in cell bodies and dendrites. Immunoprecipitation and Western blotting studies have indicated that the subunit molecular weights of brain spectrin(240/235) and (240/235E) are identical in all mammalian species. We demonstrate that when proteolysis is not completely blocked during immunoprecipitation studies, the 235 kDa subunits are converted to a 230 kDa polypeptide [brain spectrin(240/235)] and a 232 kDa polypeptide [brain spectrin(240/235E)]. Finally, we show that both the alpha and beta subunits of brain spectrin(240/235) and brain spectrin(240/235E) are antigenically distinct in every species examined. These studies indicate that previous findings on the structure, location, and function of mouse brain spectrin isoforms can now be generalized to all mammalian species.
Mots-clé
Aged, Animals, Antibody Specificity, Brain/metabolism, Cattle, Humans, Immunohistochemistry, Isomerism, Male, Mice, Rats, Spectrin/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:35
Dernière modification de la notice
20/08/2019 15:26
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