Improved immunogenicity against HIV-1 clade C antigens by using NYVAC-C with restored replication competence.

Détails

ID Serval
serval:BIB_6BB71B21AD64
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Improved immunogenicity against HIV-1 clade C antigens by using NYVAC-C with restored replication competence.
Titre de la conférence
AIDS Vaccine 2010
Auteur(s)
Mooij P., Nieuwenhuis I., Beenhakker N., Hofman S., Esteban M., Jacobs B., Kibler K., Koopman K., Bogers W.M.J.M, Pantaleo P., Heeney J.L.
Adresse
Atlanta, Georgia, United-States, September 28-October 1, 2010
ISBN
0047-2565
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
40
Série
Journal of Medical Primatology
Pages
265-266
Langue
anglais
Résumé
In order to improve the immunogenicity of currently available non-replicating pox virus HIV vaccine vectors, NYVAC was genetically modified through re-insertion of two host range genes (K1L and C7L), resulting in restored replicative capacity in human cells. In the present study these vectors, expressing either a combination of the HIV-1 clade C antigens Env, Gag, Pol, Nef, or a combination of Gal, Pol, Nef were evaluated for safety and immunogenicity in rhesus macaques, which were immunized at weeks 0, 4 and 12 either by scarification (conventional poxvirus route of immunization), intradermal or by intramuscular injection (route used in previous vaccine studies).Replication competent NYVAC-C-KC vectors induced higher HIV-specific responses, as measured by IFN- ELISpot assay, than the replication defective NYVAC-C vectors. Application through scarification only required one immunization to induce maximum HIV-specific immune responses. This method simultaneously induced relatively lower anti-vector responses. In contrast, two to three immunizations were required when the NYVAC-C-KC vectors were given by intradermal or intramuscular injection and this method tended to generate slightly lower responses. Responses were predominantly directed against Env in the animals that received NYVAC-C-KC vectors expressing HIV-1 Env, Gag, Pol, Nef, while Gag responses were dominant in the NYVAC-C-KC HIV-1 Gag, Pol, Nef immunized animals.The current study demonstrates that NYVAC replication competent vectors were well tolerated and showed increased immunogenicity as compared to replication defective vectors. Further studies are needed to evaluate the most efficient route of immunization and to explore the use of these replication competent NYVAC vectors in prime/boost combination with gp120 protein-based vaccine candidates. This studies was performed within the Poxvirus T-cell Vaccine Discovery Consortium (PTVDC) which is part of the CAVD program.
Web of science
Création de la notice
05/10/2011 9:23
Dernière modification de la notice
20/08/2019 14:25
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