Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent period.

Détails

ID Serval
serval:BIB_6AAF64480240
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent period.
Périodique
PloS one
Auteur⸱e⸱s
Rothen J., Murie C., Carnes J., Anupama A., Abdulla S., Chemba M., Mpina M., Tanner M., Lee Sim B.K., Hoffman S.L., Gottardo R., Daubenberger C., Stuart K.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
13
Numéro
6
Pages
e0199392
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: epublish
Résumé
Malaria continues to be one of mankind's most devastating diseases despite the many and varied efforts to combat it. Indispensable for malaria elimination and eventual eradication is the development of effective vaccines. Controlled human malaria infection (CHMI) is an invaluable tool for vaccine efficacy assessment and investigation of early immunological and molecular responses against Plasmodium falciparum infection. Here, we investigated gene expression changes following CHMI using RNA-Seq. Peripheral blood samples were collected in Bagamoyo, Tanzania, from ten adults who were injected intradermally (ID) with 2.5x104 aseptic, purified, cryopreserved P. falciparum sporozoites (Sanaria® PfSPZ Challenge). A total of 2,758 genes were identified as differentially expressed following CHMI. Transcriptional changes were most pronounced on day 5 after inoculation, during the clinically silent liver phase. A secondary analysis, grouping the volunteers according to their prepatent period duration, identified 265 genes whose expression levels were linked to time of blood stage parasitemia detection. Gene modules associated with these 265 genes were linked to regulation of transcription, cell cycle, phosphatidylinositol signaling and erythrocyte development. Our study showed that in malaria pre-exposed volunteers, parasite prepatent period in each individual is linked to magnitude and timing of early gene expression changes after ID CHMI.
Mots-clé
Blood Proteins/genetics, Gene Expression Profiling, Gene Expression Regulation/genetics, Humans, Malaria, Falciparum/blood, Malaria, Falciparum/genetics, Malaria, Falciparum/parasitology, Parasitemia/blood, Parasitemia/genetics, Plasmodium falciparum/isolation & purification, Plasmodium falciparum/pathogenicity, Transcriptome/genetics, Volunteers
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/02/2022 11:45
Dernière modification de la notice
23/03/2024 7:24
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