Sequence determinants of a microtubule tip localization signal (MtLS).

Détails

ID Serval
serval:BIB_6A79400E332C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Sequence determinants of a microtubule tip localization signal (MtLS).
Périodique
Journal of Biological Chemistry
Auteur⸱e⸱s
Buey R.M., Sen I., Kortt O., Mohan R., Gfeller D., Veprintsev D., Kretzschmar I., Scheuermann J., Neri D., Zoete V., Michielin O., de Pereda J.M., Akhmanova A., Volkmer R., Steinmetz M.O.
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
287
Numéro
34
Pages
28227-28242
Langue
anglais
Résumé
Microtubule plus-end-tracking proteins (+TIPs) specifically localize to the growing plus-ends of microtubules to regulate microtubule dynamics and functions. A large group of +TIPs contain a short linear motif, SXIP, which is essential for them to bind to end-binding proteins (EBs) and target microtubule ends. The SXIP sequence site thus acts as a widespread microtubule tip localization signal (MtLS). Here we have analyzed the sequence-function relationship of a canonical MtLS. Using synthetic peptide arrays on membrane supports, we identified the residue preferences at each amino acid position of the SXIP motif and its surrounding sequence with respect to EB binding. We further developed an assay based on fluorescence polarization to assess the mechanism of the EB-SXIP interaction and to correlate EB binding and microtubule tip tracking of MtLS sequences from different +TIPs. Finally, we investigated the role of phosphorylation in regulating the EB-SXIP interaction. Together, our results define the sequence determinants of a canonical MtLS and provide the experimental data for bioinformatics approaches to carry out genome-wide predictions of novel +TIPs in multiple organisms.
Mots-clé
Amino Acid Motifs, Arabidopsis/chemistry, Arabidopsis/genetics, Arabidopsis Proteins/chemistry, Arabidopsis Proteins/genetics, Humans, Microtubule Proteins/chemistry, Microtubule Proteins/genetics, Microtubules/chemistry, Microtubules/genetics, Protein Array Analysis, Protein Binding, Protein Sorting Signals/physiology, Schizosaccharomyces/chemistry, Schizosaccharomyces/genetics, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/01/2013 15:59
Dernière modification de la notice
20/08/2019 14:25
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