Monocyte adhesion to activated aortic endothelium: role of L-selectin and heparan sulfate proteoglycans

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_6924C9E33A88
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Monocyte adhesion to activated aortic endothelium: role of L-selectin and heparan sulfate proteoglycans
Périodique
Journal of Cell Biology
Auteur(s)
Giuffre  L., Cordey  A. S., Monai  N., Tardy  Y., Schapira  M., Spertini  O.
ISSN
0021-9525 (Print)
Statut éditorial
Publié
Date de publication
02/1997
Volume
136
Numéro
4
Pages
945-956
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb 24
Résumé
This study examines the role of L-selectin in monocyte adhesion to arterial endothelium, a key pathogenic event of atherosclerosis. Using a nonstatic (rotation) adhesion assay, we observed that monocyte binding to bovine aortic endothelium at 4 degrees C increased four to nine times upon endothelium activation with tumor necrosis factor (TNF)-alpha. mAb-blocking experiments demonstrated that L-selectin mediates a major part (64 +/- 18%) of monocyte attachment. Videomicroscopy experiments performed under flow indicated that monocytes abruptly halted on 8-h TNF-alpha-activated aortic endothelium, approximately 80% of monocyte attachment being mediated by L-selectin. Flow cytometric studies with a L-selectin/IgM heavy chain chimeric protein showed calcium-dependent L-selectin binding to cytokine-activated and, unexpectedly, unactivated aortic cells. Soluble L-selectin binding was completely inhibited by anti-L-selectin mAb or by aortic cell exposure to trypsin. Experiments with cycloheximide, chlorate, or neuraminidase showed that protein synthesis and sulfate groups, but not sialic acid residues, were essential for L-selectin counterreceptor function. Moreover, heparin lyases partially inhibited soluble L-selectin binding to cytokine-activated aortic cells, whereas a stronger inhibition was seen with unstimulated endothelial cells, suggesting that cytokine activation could induce the expression of additional ligand(s) for L-selectin, distinct from heparan sulfate proteoglycans. Under flow, endothelial cell treatment with heparinase inhibited by approximately 80% monocyte attachment to TNF-alpha-activated aortic endothelium, indicating a major role for heparan sulfate proteoglycans in monocyte-endothelial interactions. Thus, L-selectin mediates monocyte attachment to activated aortic endothelium, and heparan sulfate proteoglycans serve as arterial ligands for monocyte L-selectin.
Mots-clé
Animals Aorta Cattle Cell Adhesion/drug effects Cells, Cultured Cytokines/pharmacology Endothelium, Vascular/drug effects/*physiology Heparan Sulfate Proteoglycans Heparitin Sulfate/*physiology Humans Kinetics L-Selectin/biosynthesis/*physiology Ligands Monocytes/drug effects/*physiology Proteoglycans/*physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:27
Dernière modification de la notice
20/08/2019 15:24
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