Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes.

Détails

ID Serval
serval:BIB_69022E3042AF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes.
Périodique
European Journal of Cell Biology
Auteur(s)
Obarzanek-Fojt M., Favre B., Kypriotou M., Ryser S., Huber M., Hohl D.
ISSN
1618-1298[electronic], 0171-9335[linking]
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
90
Numéro
4
Pages
279-290
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The homeodomain-only protein (HOP) contains an atypical homeodomain which is unable to bind to DNA due to mutations in residues important for DNA binding. Recently, HOP was reported to regulate proliferation/differentiation homeostasis in different cell types. In the present study, we performed transcriptional profiling of cultured primary human keratinocytes and noted a robust induction of HOP upon calcium-induced cell differentiation. Immunohistochemistry of human skin localized HOP to the granular layer in the epidermis. Overexpression of HOP using a lentiviral vector up-regulated FLG and LOR expression during keratinocyte differentiation. Conversely, decreasing HOP expression using small interfering RNA markedly reduced the calcium-induced expression of late markers of differentiation in vitro, with the most prominent effect on profilaggrin (FLG) mRNA. Moreover, mRNA levels of profilaggrin and loricrin were downregulated in the epidermis of HOP knockout mice. Analysis of skin disorders revealed altered HOP expression in lichen planus, psoriasis and squamous cell carcinoma (SCC). Our data indicate that HOP is a novel modulator of late terminal differentiation in keratinocytes.
Pubmed
Web of science
Création de la notice
10/03/2011 9:47
Dernière modification de la notice
20/08/2019 15:24
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