Selective toxicity of the general anesthetic propofol for GABAergic neurons in rat brain cell cultures.

Détails

ID Serval
serval:BIB_68F5B67016CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Selective toxicity of the general anesthetic propofol for GABAergic neurons in rat brain cell cultures.
Périodique
Journal of Neuroscience Research
Auteur(s)
Honegger P., Matthieu J.M.
ISSN
0360-4012 (Print)
ISSN-L
0360-4012
Statut éditorial
Publié
Date de publication
1996
Volume
45
Numéro
5
Pages
631-636
Langue
anglais
Résumé
The intravenous, short-acting general anesthetic propofol was applied to three-dimensional (aggregating) cell cultures of fetal rat telencephalon. Both the clinically used formulation (Disoprivan, ICI Pharmaceuticals, Cheshire, England) and the pure form (2,6-diisopropylphenol) were tested at two different periods of brain development: immature brain cell cultures prior to synaptogenesis and at the time of intense synapses and myelin formation. At both time periods and for clinically relevant concentrations and time of exposure (i.e., concentrations > or = 2.0 micrograms/ml for 8 hr), propofol caused a significant decrease of glutamic acid decarboxylase activity. This effect persisted after removal of the drug, suggesting irreversible structural changes in GABAergic neurons. The gamma-aminobutyric acid type A (GABAA) blocking agents bicuculline and picrotoxin partially attenuated the neurotoxic effect of propofol in cultures treated at the more mature phase of development. This protective effect was not observed in the immature brain cells. The present data suggest that propofol may cause irreversible lesions to GABAergic neurons when given at a critical phase of brain development. In contrast, glial cells and myelin appeared resistant even to high doses of propofol.
Mots-clé
Anesthetics, General/toxicity, Animals, Cells, Cultured, DNA/metabolism, Glutamate Decarboxylase/metabolism, Myelin Basic Protein/metabolism, Nerve Tissue Proteins/metabolism, Neurons/drug effects, Neurons/enzymology, Parasympathetic Nervous System/cytology, Parasympathetic Nervous System/drug effects, Propofol/toxicity, Rats, Telencephalon/cytology, Telencephalon/enzymology, gamma-Aminobutyric Acid/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 15:24
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