Xkid chromokinesin is required for the meiosis I to meiosis II transition in Xenopus laevis oocytes

Détails

ID Serval
serval:BIB_685836A44691
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Xkid chromokinesin is required for the meiosis I to meiosis II transition in Xenopus laevis oocytes
Périodique
Nat Cell Biol
Auteur⸱e⸱s
Perez L. H., Antonio C., Flament S., Vernos I., Nebreda A. R.
ISSN
1465-7392 (Print)
1465-7392
Statut éditorial
Publié
Date de publication
10/2002
Volume
4
Numéro
10
Pages
737-42
Langue
anglais
Notes
Perez, Laurent H
Antonio, Celia
Flament, Stéphane
Vernos, Isabelle
Nebreda, Angel R
Journal Article
England
Nat Cell Biol. 2002 Oct;4(10):737-42. doi: 10.1038/ncb850.
Résumé
Xkid chromokinesin is required for chromosome alignment on the metaphase plate of spindles formed in Xenopus laevis egg extracts. We have investigated the role of Xkid in Xenopus oocyte meiotic maturation, a progesterone-triggered process that reinitiates the meiotic cell cycle in oocytes arrested at the G2/M border of meiosis I. Here we show that Xkid starts to accumulate at the time of germinal vesicle breakdown and reaches its largest quantities at metaphase II in oocytes treated with progesterone. Both germinal vesicle breakdown and spindle assembly at meiosis I can occur normally in the absence of Xkid. But Xkid-depleted oocytes cannot reactivate Cdc2/cyclin B after meiosis I and, instead of proceeding to meiosis II, they enter an interphase-like state and undergo DNA replication. Expression of a Xkid mutant that lacks the DNA-binding domain allows Xkid-depleted oocytes to complete meiotic maturation. Our results show that Xkid has a role in the meiotic cell cycle that is independent from its role in metaphase chromosome alignment.
Mots-clé
Animals, Apoptosis/genetics, CDC2 Protein Kinase/genetics/metabolism, Cell Cycle Proteins/drug effects/genetics, Cell Differentiation/drug effects/genetics, Chromosome Segregation/drug effects/*genetics, Cyclin B/genetics/metabolism, DNA Replication/drug effects/genetics, DNA-Binding Proteins/biosynthesis/deficiency/*genetics, Female, Kinesin/biosynthesis/deficiency/*genetics, Meiosis/drug effects/*genetics, Nuclear Proteins/biosynthesis/deficiency/*genetics, Oligoribonucleotides, Antisense, Oocytes/drug effects/*growth & development/metabolism, Protein Structure, Tertiary/drug effects/genetics, *Xenopus Proteins, Xenopus laevis/embryology/*genetics
Création de la notice
04/09/2020 19:03
Dernière modification de la notice
07/09/2020 5:26
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